The preparation methods of α-arbutin (Alpha arbutin, CAS No.: 84380-01-8) mainly include biotransformation method and enzymatic synthesis method.
1. Biotransformation
(1) Plant cell tissue as a bioreactor to synthesize α-arbutin The method utilizes the glycosylase in the organism to convert exogenous hydroquinone into arbutin. The yield is higher and the stereoselectivity is better. Plant cell tissue as a bioreactor to synthesize arbutin, the key lies in the selection of plant tissue culture medium and the determination of transformation conditions. But different kinds of plant tissue culture have great influence on the conversion rate of hydroquinone.
(2) Synthesis of α-arbutin by microbial cell transformation method The method is mainly to selectively synthesize α-arbutin through the catalysis of glucose and hydroquinone by different microbial metabolic enzymes.
2. Enzymatic synthesis
(1) Synthesis of α-arbutin by transglycosylation reaction catalyzed by glycosidase Glycosidase is mainly used to cleave glycosidic bonds in organisms, but under certain conditions, it can catalyze the synthesis of glycosides through reverse hydrolysis and transglycosylation reactions. Synthesis of α-arbutin by transglycosylation reaction catalyzed by glycosidase.
(2) α-Cyclodextrin glucosyltransferase catalyzed the synthesis of α-arbutin Using hydroquinone and maltodextrin as substrates, a two-step enzymatic reaction system of α-cyclodextrin glucosyltransferase and amyloglucosidase was used to catalyze the synthesis of α-arbutin.