Melatonin is one of the hormones secreted by the pineal gland in the brain. It can inhibit the activity of the hypothalamus-pituitary-target axis, reduce the concentration of cortisol in the blood, regulate people's natural sleep, overcome sleep disorders, and improve sleep quality. The secretion of melatonin in the human body has the law of day low and night high, generally reaching a peak around 2 am. As people age, melatonin secretion will be insufficient, resulting in sleep disorders and a series of dysfunctions. Therefore, supplementing melatonin in vitro can maintain the melatonin level in the body in a young state, adjust and restore the circadian rhythm, deepen sleep, improve sleep quality, improve the functional state of the body and delay aging.